Na-Sal enhances growth factor withdrawal-induced and daunorubicin-induced apoptosis of TF-1 cells. (A) TF-1 cells were grown in the presence or absence of GM-CSF and treated with 3 mmol/L Na-Sal. Viability was determined by trypan blue dye exclusion at the times indicated. (B) TF-1 cells were treated with daunorubicin (Dauno) alone (0.1 μg/mL or 0.5 μg/mL), Na-Sal alone (2 mmol/L) or the combination of daunorubicin (0.1 μg/mL) and 2 mmol/L Na-Sal for the times indicated. Viability was determined using the MTT assay. These experiments were repeated three times with similar results. Results of a representative experiment are shown.