Fig. 5.
Sequence analysis of β3 cDNA from 24/12 or AM-1 cells. (A) The results of sequencing using an antisense primer are shown. The same results were obtained using a sense primer for sequencing (data not shown). The mutated nucleotides and the changed amino acid are in bold. (B) Amino acid alignment around the mutated site is shown. T562 in β3 and corresponding amino acids in other β integrins are in bold. NRT (underlined) is a consensus sequence for N-glycosylation. Amino acid sequences were obtained from Wippler et al,44 Mimura et al,53and Ransom et al.54
