Effect of proteinase inhibitors on IL-8–directed neutrophil chemotaxis. Purified peripheral blood PMNs were untreated (▪) or preincubated with 1,10-phenanthroline (0.5 mmol/L, ▨), EDTA (5 mmol/L, ░), or bestatin (100 μmol/L, □) for 30 minutes at 37°C followed by the addition of LPS (100 ng/mL), TNF- (50 ng/mL), or IL-8 (500 ng/mL) for 1 hour at 37°C. The migration assay was then performed. The data represent a single experiment from 4 performed. Results are the mean ± SEM migrated cells counted from three high-powered fields (400×) obtained in three replicates. *Statistical significance (P < .05) using one-way ANOVA for control untreated v treated groups.