Ptyr 155 SHIP2 selectively binds to PTB domain of SHC and the SH3 domain of Abl. NP-40 lysates from 10 × 10⁶ (A) K562, (B) R10⁺, and (C) M07 cells were mixed with GST-fusion protein immobilized on glutathione-agarose beads for 2 hours at 4°C. Precipitated proteins were resolved by SDS-PAGE and immunoblotted with 4G10. The filter was stripped and immunoblotted sequentially with anti-SHIP2 and anti-SHIP1 antibodies.