SHIP2 is a target for tyrosine phosphorylation after stimulation of M07 cells with SCF, IL-3, and GM-CSF. Starved M07 cells were left untreated (U) or treated with SCF, GM-CSF, or IL-3 (100 ng/mL) for 5 minutes at 37°C and then lysed. NP-40 lysates (10 × 10⁶ cell equivalents) were immunoprecipitated with anti-SHIP2 antibody. Immunoprecipitates were separated by SDS-PAGE, transferred to Immobilon, and immunoblotted with 4G10. Blot was reprobed sequentially with anti-SHIP2 and anti-SHC antibodies.