Expression of IκB at mRNA (A) and protein (B) levels in primary leukemic cells from ATL patients and normal healthy subjects. (A) Total RNA was extracted from PBMC and assessed for IκB, IL-2R, or GAPDH mRNA expression. A representative Northern blot is shown from patients with ATL and healthy subjects (normal). (B) PBMC obtained from patients with ATL or healthy subjects (normal) were assessed for IκB by Western blot analysis.