Fig. 6.
![Fig. 6. NF-κB binding activity in various cell lines. Nuclear extracts were prepared from cells. Nuclear extracts (5 μg) were incubated with a [32P]-radiolabeled oligonucleotide that corresponds to the κB sequence of the IL-2R gene and analyzed by EMSA. (B) In super-shift assays, antibody specific for each NF-κB subunit (indicated above the lanes) was incubated with MT-2 and TL-Om1 extracts before probe addition.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/7/10.1182_blood.v93.7.2360/5/blod4071006abw.jpeg?Expires=1735496504&Signature=jzJyUT92yI09mmsNWWjrK4jPxOvtbp~l-D5sWGyxxbmaW5wVEX2n0M2MRiHaeWWpcnpGq4RKUNY58RQxniZ1DUoEaoY5sd~nEJDRAEyDLz2DqAmb-bcgtjsLvZVb2RxzIXoptx4GYjDQ9Z9lNLtjzdxTsmxvIl-T0UJuRViJ~4cM2aDwBZBxzbran-CE4ct~kbANG0w~OhQGBBL1DpYbfvMyfc7lhfveX~GETReGDNLaUOXemKix2JXqIKmJVnvR51K8S38zLDHkwyTodbd2exEi~A03uchphrCQVMChee0VIMzABn1Q6aQxJk4tw8iYydZrn2-teIFQ~1AOPGOYUQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
NF-κB binding activity in various cell lines. Nuclear extracts were prepared from cells. Nuclear extracts (5 μg) were incubated with a [32P]-radiolabeled oligonucleotide that corresponds to the κB sequence of the IL-2R gene and analyzed by EMSA. (B) In super-shift assays, antibody specific for each NF-κB subunit (indicated above the lanes) was incubated with MT-2 and TL-Om1 extracts before probe addition.
