Schematic illustration of the localization of the primers for genotyping of C₈₀₇T dimorphism (top). Allele-specific amplification (PCR-SSP) was performed with intronic common sense primer (92V) and antisense primers C₈₀₇ or T₈₀₇ (96C or 96T). Representative results of GPIa genotypes determined by PCR-SSP of three individuals: homozygous CC (lanes 2), heterozygous CT (lanes 3), and homozygous TT (lanes 4). Genomic DNA were amplified using specific primer for C₈₀₇ (left panel) or for T₈₀₇ allele (right panel) and were analyzed on 1.8% agarose gel electrophoresis. The GPIa bands represent the allele-specific PCR product and the HGH bands the internal control. DNA size standards V (Boehringer Mannheim) were used as standard (lanes 1). Negative controls are shown in lanes 5.