American Society of Hematology

Fig. 2.

$Fig. 2. TNF-–induced Bcl-2 family protein redistribution. Cells were treated with 250 U/mL TNF- for 3 hours. Whole cell lysate and cellular fractions were prepared, and the same amount of protein (50 μg/lane) was analyzed by Western blotting. Ratios of mitochondrial Bcl-2 family proteins after exposure to TNF- are CEM:CEM/VLB100 for Bcl-XS = 33:67 (1:2); CEM:CEM/VLB100 for Bad = 36:64 (1:1.8); and for Bax = 12:88 (1:7.3). At the whole cell level, the CEM cell line the ratio of control:TNF for Bcl-XS = 37:63 (1:1.7); control:TNF for Bad = 29:71 (1:2.4); and the CEM/VLB100cell line the ratio of control:TNF for Bcl-2 = 59:41 (1.4:1).$

TNF-–induced Bcl-2 family protein redistribution. Cells were treated with 250 U/mL TNF- for 3 hours. Whole cell lysate and cellular fractions were prepared, and the same amount of protein (50 μg/lane) was analyzed by Western blotting. Ratios of mitochondrial Bcl-2 family proteins after exposure to TNF- are CEM:CEM/VLB₁₀₀ for Bcl-X_S = 33:67 (1:2); CEM:CEM/VLB₁₀₀ for Bad = 36:64 (1:1.8); and for Bax = 12:88 (1:7.3). At the whole cell level, the CEM cell line the ratio of control:TNF for Bcl-X_S = 37:63 (1:1.7); control:TNF for Bad = 29:71 (1:2.4); and the CEM/VLB₁₀₀cell line the ratio of control:TNF for Bcl-2 = 59:41 (1.4:1).

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