Fig. 5.
Fig. 5. Differentiation kinetics of the second wave of thymocyte progenitors (E13) into E10, E13, and E15 recipients. After adoptive transfer of E13 H.B19ov+ BM (10 × 106cells) into E10, E13, and E15 H.B19ov− embryos, the donor cells were examined for T-cell expression 7 days and 10 days after injection. Thymocytes of recipients were analyzed by immunofluorescence flow cytometry: ov, TCRγδ and ov, TCR Vβ1. Each dot plot presents 50,000 events for the gated thymocyte population. In the three types of recipients, differentiation kinetics were similar because donor γδ thymocytes were detected 7 days after injection, whereas β donor thymocytes were detected 10 days after injection.

Differentiation kinetics of the second wave of thymocyte progenitors (E13) into E10, E13, and E15 recipients. After adoptive transfer of E13 H.B19ov+ BM (10 × 106cells) into E10, E13, and E15 H.B19ov embryos, the donor cells were examined for T-cell expression 7 days and 10 days after injection. Thymocytes of recipients were analyzed by immunofluorescence flow cytometry: ov, TCRγδ and ov, TCR Vβ1. Each dot plot presents 50,000 events for the gated thymocyte population. In the three types of recipients, differentiation kinetics were similar because donor γδ thymocytes were detected 7 days after injection, whereas β donor thymocytes were detected 10 days after injection.

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