Fig. 4.
Antigen uptake capability of CBDC. Lin−(CD3, CD11b, CD14, CD16, CD19, CD34, CD56, and glycophorin A negative) enriched CB cells (A), SRBC− cells (B), and SRBC+ cells (C) were pulsed for 60 minutes at 4°C (filled histograms) or 37°C (open histograms) with FITC-conjugated peptide (upper panel) or BSA (lower panel) in the presence of MoAb to HLA-DR (A), CD14 (B), and CD3 (C), respectively. FITC fluorescence of gated HLA-DR+, CD14+, and CD3+ cells is shown on an arbitrary 4-log scale.