Effect of echicetin and echistatin on botrocetin-enhanced HUVEC adhesion to immobilized vWF. A 96-well microplate was coated with vWF, incubated overnight at 4°C, and then blocked with 3% BSA. HUVECs, after detachment, were applied to the microplate in the absence (•) and presence (▪) of 10 μg/mL botrocetin during the 2-hour incubation period. Various concentrations of echicetin (A) and echistatin (B) were added to the HUVECs before their application to the plate. In (B), the difference between samples examined in the presence and absence of botrocetin was significant at P < .05 for all concentrations of echistatin studied. In (A) (for echicetin), this statistically significant difference was not observed except for the control sample.