(A) HUVEC adhesion to immobilized ligands. A 96-well microplate was coated with various ligands of 1% BSA (lane 1), 15 μg/mL vWF (lane 2), 50 μg/mL echicetin (lane 3), 50 μg/mL alboaggregin A (lane 4), and 50 μg/mL alboaggregin B (lane 5) overnight at 4°C. After blocking with 3% BSA, 100-μL aliquots of 2 × 10⁵ HUVECs/mL were added to each well. After 2 hours of incubation at 37°C in the presence of 5% CO₂, the attached cells were fixed and stained with methylene blue. The cells were then lysed and an OD reading was taken. The data represent the mean and SD of OD readings from five individual experiments. (B) Adhesion of transfected CHO cells to immobilized ligands. A 96-well microplate was coated with various ligands (BSA [lane 1], vWF [lane 2], echicetin [lane 3], alboaggregin A [lane 4], and alboaggregin B [lane 5]) and blocked with BSA. Aliquots (100 μL) of CHOβIX cells at 2 × 10⁵ cells/mL were added to each well. Wells coated with vWF received cells that had been treated with 10 μg/mL botrocetin. Cells were incubated on the plate for 2 hours at 37°C in the presence of 5% CO₂. CHOβIX cells and control (untransfected) CHO cells showed baseline adhesion to immobilized vWF that was comparable to the adhesion level of CHOβIX cells to BSA (lane 1).