HUVECs adherent to various substrata examined by fluorescence microscopy. HUVECs were allowed to adhere to different ligands on chamber slides. After fixation with formaldehyde and staining with sulforhodamine and DAPI, the slides were examined by fluorescence microscopy. Details of the nuclei and the cytoplasm were better visualized using this dual stain. HUVECs adherent to vWF (A) and alboaggregin B (B) demonstrated extensive cell spreading, whereas HUVECs adherent to echicetin (C) and echistatin (D) showed minimal cell spreading.