H₂O₂ mimics GM-CSF–induced G1-S phase transition, early gene expresion, and random transwell migration in MO7e cells. (A) Gene expression was analyzed using mRNA from MO7e cells left untreated (CTRL) or stimulated for the indicated times with GM-CSF (20 ng/mL) or H₂O₂ (PEROX, 250 μmol/L). The expression of c-FOS and G3PDH was detected by Northern blotting using specific probes. (B) MO7e cells were either left untreated or treated with GM-CSF (20 ng/mL) or H₂O₂ (PEROX, 50 μmol/L). Cell cycle analysis was performed using propidium iodide at the indicated time points and the samples were analyzed by flow cytometry. (C) MO7e cells were either left untreated or treated with GM-CSF, SF, or H₂O₂ (PEROX) as indicated and used for a random transwell migration assay. The number of viable cells in the lower chamber was determined after 5 hours of migration by trypan blue exclusion. The error bars indicate the SEM (n = 4).