QAS-PCR results of HLA DR and DQ amplification for samples collected from recipients B (A) and D (B). Pre-tx and serial post-tx samples collected from female trauma tx recipients B and D were amplified with a panel of primer pairs specific to HLA DR (1, 2, 4, 7) and DQ (2, 4, 5, 6, 7, 8) and hybridized with specific probes. The results of DR2 and DQ6 were positive and DQ4 and DQ7 negative at all time points, and are not shown for recipient B; the results of DR1, DR2, DQ4, DQ5, and DQ7 were negative at all time points and are not shown for recipient D.