Effect of the anti-CD18 MoAb MEM48 on the GM-CSF–stimulated neutrophil-mediated Lym-1 antibody-dependent cytolysis. 51Cr-labeled Raji cells were at 2 × 104. The neutrophil-Raji cell ratio was 20:1. Lym-1 and GM-CSF were at 10 μg/mL and 1 ng/mL, respectively. The incubation time was 14 hours. (A) The lysis was 21.94 ± 11.30 (mean ± 1 SD, n = 10) with a median of 23.00 (confidence interval 95%, 13.86 to 30.02) in the absence of MEM48 and 7.30 ± 4.82 (mean ± 1 SD, n = 10) with a median of 7.60 (confidence interval 95%, 3.85 to 10.75) in the presence of 1 μg/mL MEM48. Cytolysis in the absence versus that in the presence of 1 μg/mL MEM48 was P = .0052. (B) The lysis was 23.58 ± 12.64 (mean ± 1 SD, n = 13) with a median of 26.90 (confidence interval 95%, 15.94 to 31.22) in the absence of MEM48 and 10.29 ± 6.96 (mean ± 1 SD, n = 13) with a median of 10.30 (confidence interval 95%, 6.08 to 14.50) in the presence of 4 μg/mL MEM48. Cytolysis in the absence versus that in the presence of 4 μg/mL MEM48 was P = .0061.
