Effect of NADG and D-mannose on the GM-CSF–stimulated neutrophil-mediated Lym-1 antibody-dependent cytolysis.51Cr-labeled Raji cells were at 2 × 104. The neutrophil-Raji cell ratio was 20:1. Lym-1 and GM-CSF were at 10 μg/mL and 1 ng/mL, respectively. The incubation time was 14 hours. (A) The lysis was 24.15 ± 7.72 (mean ± 1 SD, n = 6) with a median of 24.75 (confidence interval 95%, 10.06 to 32.25) in the absence of NADG and 6.08 ± 3.70 (mean ± 1 SD, n = 6) with a median of 6.20 (confidence interval 95%, 2.19 to 9.97) in the presence of 100 mmol/L NADG. Cytolysis in the absence versus that in the presence of NADG wasP = .0022. (B) The lysis was 28.12 ± 4.81 (mean ± 1 SD, n = 4) with a median of 29.70 (confidence interval 95%, 20.46 to 35.78) in the absence of D-mannose and 3.30 ± 1.31 (mean ± 1 SD, n = 4) with a median of 3.15 (confidence interval 95%, 1.22 to 5.38) in the presence of 100 mmol/L D-mannose. Cytolysis in the absence versus that in the presence of D-mannose was P = .028.
