SSCP analysis of plasminogen gene exon 2 in patient no. 1, his healthy parents, and his healthy brother and in healthy subject H. Radiolabeled single-stranded PCR products of plasminogen exon 2 were electrophoresed in a nondenaturing 5% polyacrylamide gel as described in Materials and Methods. Lane 1, healthy control (C); lane 2, father of patient no. 1; lane 3, mother of patient no. 1; lane 4, patient no. 1 with ligneous conjunctivitis; lane 5, brother of patient no. 2; lane 6, healthy subject H; lane 7, healthy control. dsDNA, double-stranded DNA.