Inhibition of CD95 signaling does not affect antitumor drug-mediated cell death. Jurkat (▩) or Jurkat-R () cells (3 × 10⁴ per well) were preincubated with (A) medium, (B) GST-CD95 (100 μg/mL), or (C) GST (100 μg/mL) for 1 hour, and then treated with either anti-CD95 (20 ng/mL), daunorubicin (Dauno; 5 μg/mL), doxorubicin (Doxo; 1 μg/mL), etoposide (Etopo; 25 μg/mL), or mitomycin C (Mito; 25 μg/mL). Apoptosis was assessed after 24 hours by measuring phosphatidylserine exposure by annexin-V-FITC-staining and flow cytometry.