PMA induces NF-κB specific DNA-binding in human CD34⁺CD19⁻ bone marrow cells. To confirm specificity and identify which Rel family members may be binding to the NF-κB consensus sequence, super-shift EMSA were performed with antibodies specific for human p50, p65, p52, rel B, and c-rel. The CD34⁺CD19⁻ cells were 96.8% pure for this representative experiment.