The RT-PCR products of chymase genes from total RNA extracted from mBMMC cultures. Initial dilutions of the RNA template before reverse transcription are indicated (1, 0.1, 0.01, and 0.001 μg/mL). (A) shows the RT-PCR products using primer sets specific for the mMCP-1, mMCP-2, mMCP-4, mMCP-5, and G3PDH genes as indicated. Target RNA was from mBMMC cultured in WEHI/rrSCF alone (W/S), in rhTGF-β₁ (1 ng/mL)/rmIL-9 (5 ng/mL)/WEHI (15%)/rrSCF (50 ng/mL) (T/I/W/S), in rmIL-9/WEHI/rrSCF (I/W/S) (day 14, as described in Fig 3), or in rmIL-9/WEHI/rrSCF (I/W/S) in the presence of anti–TGF-β antibody (TGFβ Ab) or a control antibody (Con Ab) as indicated (cultures described in Fig 6). Transcription of mMCP-1 and mMCP-2 is suppressed by the presence of anti–TGF-β antibodies, whereas transcripts for mMCP-4 and mMCP-5 are unaffected. Note the much higher level of transcription of mMCP-1 and mMCP-2 in the culture supplemented with TGF-β₁. (B) shows the RT-PCR products using primer sets specific for the TGF-β₁ and G3PDH genes as indicated. Target RNA was as described for (A). The transcripts for TGF-β₁ appear to be unaffected by the culture conditions or the presence of anti–TGF-β antibodies.