Selective chemoattraction of DC precursors derived from murine HPCs by SLC and its regulation by TGF-β1. (A) Selective migration of DC precursors from GM-CSF+SCF– and GM-CSF+SCF+TNF-–stimulated HPCs was abrogated by TGF-β1. The profiles in the uppermost row indicate the preloaded populations, and those in the rest of the rows indicate the population in the lower chamber after migration assay. (B) TGF-β1–induced macrophages (at day 13; ▩) were less sensitive to SLC than HPC stimulated with GM-CSF+SCF (▪). (C) LC-like mature DCs successively generated with GM-CSF+TNF- from TGF-β1–induced macrophages (at day 13) exhibited high selective migration toward SLC. The values in (B) and (C) represent the mean value ± SD of percentage. The results are representative of more than three experiments.