ICE/caspase-1 is upregulated and activated by IFNγ in human ECFC. Day-6 cells were incubated in liquid medium or medium containing 500 U/mL of IFNγ for 24 to 96 hours and immunoblot analysis was performed using whole cell lysates and antibody to caspase-1. An increase in caspase-1 and its activation fragments was observed in IFNγ-treated cells by 24 hours.