Differential binding of nuclear proteins to the −401 and −402 polymorphic sites. (A) EMSA of nuclear extract derived from HepG2 cells bound to a 30 base pair DNA fragment containing either the −402A site (lanes 1 and 2), the wild-type (WT) site (lane 3), or the −401T site (lane 4) of the FVII promoter. Arrows 1 and 2 denote the allele specific factors. Lane 1, without extract; lanes 2 to 4, 0.20 mg/mL HepG2 extract. (B and C) The relative intensity of the protein-DNA complexes 1 and 2 obtained with the three DNA fragments were quantified in five independent experiments. Bars indicate mean values and standard deviations. The statistical significance of differences was determined by Student’s paired t-test.