Immunofluorescence analysis of CECs. (A, B, and C) CECs isolated from donors with AMI or UA. Cells of (D) are mixed endothelial and smooth muscle cells of human umbilical vein for control. All are stained for both intracellular vWF (red) and smooth muscle-specific  actin (green). Round- and spindle-shaped cells (A and B), cell sheets (C), and umbilical vein endothelial cells (D) show the same granular pattern of vWF expression. The presence of smooth muscle-specific  actin is detected only in control smooth muscle cells (D). Nuclei are counterstained red with propidium iodide. The green halo around beads comes from anti-mouse FITC-secondary antibody bound on the S-endo 1-coated beads.