Influence of CD43 on cord blood CD34⁺ cell subsets. (A) Gates used to select cord blood CD34⁺subpopulations based on CD38 expression. Cells were sorted by FACSstar Plus after MACS preenrichment. R1: CD34⁺CD38^−/low cells; R2: CD34⁺CD38^low cells; R3: CD34⁺CD38^hi cells. (B) The effect of CD43 engagement on adhesion of CD34⁺ subpopulations to immobilized fibronectin. Cells were stimulated with isotype-matched control IgG or anti-CD43 MoAb (clone DF-T1) followed by goat antimouse IgG, then subjected to adhesion assay. Data represent the mean ± SE from three separate experiments with triplicate samples for each group in every experiment. *P < .05 versus control. (□), control; (▪) CD43-stimulated. (C) Expression of CD38 and CD43 on the CD34⁺ cell population. Three-color analysis was performed on the cells isolated from cord blood by MACS. Expression of CD38 and CD43 was analyzed on the gated CD34⁺ cell population by FACScan.