Effect of inhibitors on CD43-stimulated MO7e cell adhesion to fibronectin. (A) Cells were pretreated with 1 μmol/L wortmannin, 0.2 μg/mL chorea toxin, 50 μmol/L HA1004, or 50 μmol/L H7 for 30 minutes or 1 μg/mL herbimycin A for 18 hours at 37°C. Then, cells were cross-linked with anti-CD43 MoAb (clone DF-T1) and subjected to adhesion assay. *P < .01 versus control, **P < .01 versus CD43-stimulated adhesion without inhibitors. (□), control; (▩), CD43-stimulated; (B) Cells pretreated with 1 μmol/L wortmannin for 30 minutes at 37°C were cross-linked with anti-CD43 MoAb (clone DF-T1) or treated with 10 ng/mL steel factor and subjected to adhesion assay. *P < .01 versus steel factor-stimulated adhesion without wortmannin. (□), control; (▩), CD43-stimulated; (▪), steel factor-stimulated. (C) Cells were pretreated with U73122 at the indicated concentrations for 30 minutes at 37°C. Then, cells were cross-linked with anti-CD43 MoAb (clone DF-T1) and subjected to adhesion assay. *P < .05, **P< .001 versus CD43-stimulated adhesion without U73122. (○), control; (◍), CD43-stimulated; (•) pretreated with U733343 (2 μmol/L) before CD43 cross-linking; Data represent the mean ± SE of triplicate assays for one representative experiment of three reproducible experiments.