Fig. 1.
![Fig. 1. Epo but not osmotic shock or heat shock lead to activation of HPK1 kinase activity. (A) In vitro HPK1 autophosphorylation in Epo-dependent FD-EPO cells (A) and in Epo-responsive SKT6 cells (B). HPK1 was immunoprecipitated using a HPK1-specific antibody after Epo stimulation at the indicated time points (lanes 1 through 6) or after 0.35 mol/L NaCl treatment for 30 minutes (lane 7) or heat shock at 42°C for 30 minutes (lane 8). In vitro kinase assays were performed in the presence of [γ-32P]ATP. The arrow indicates the autophosphorylated HPK1, and the band below is nonspecific.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/10/10.1182_blood.v93.10.3347.410k06_3347_3354/5/blod41006001w.jpeg?Expires=1735500554&Signature=cvYR8HLxLWQaMY5hA5MTalYtKhqjvfDxHwobI81IW5Q6OShK98ECeavnDBgfmM3qkG5LYj404ldNYQ8oyv9OKeXvzi0matTdxdu6-DgBN9GICgawNCj4jRybousifWWmowypEJRWxGClJtdfYHVNR7Y-xAv2YcRqx2xvVtDyUdfAQE0hAiXTkaOVLis9u8X7bgC1RxBPO-E7C4-BvzNXlYKoFA6yVXAuIqW4abmTEjhZI2GZ3DwIaFxKoNtCX4uqJG0sSmHrvVAaOqOLYnVJOIhUO4VxVL1QiePSttjTDlxGZwk~i3Nu4K7SLljOHSeMoHgEPYKbrqbWtI2J5jC0Cg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Epo but not osmotic shock or heat shock lead to activation of HPK1 kinase activity. (A) In vitro HPK1 autophosphorylation in Epo-dependent FD-EPO cells (A) and in Epo-responsive SKT6 cells (B). HPK1 was immunoprecipitated using a HPK1-specific antibody after Epo stimulation at the indicated time points (lanes 1 through 6) or after 0.35 mol/L NaCl treatment for 30 minutes (lane 7) or heat shock at 42°C for 30 minutes (lane 8). In vitro kinase assays were performed in the presence of [γ-32P]ATP. The arrow indicates the autophosphorylated HPK1, and the band below is nonspecific.
