Experimental design for morphological analysis of CD34⁺CD38⁺ cell-derived colonies. The clone-sorted CD34⁺CD38⁺ cells were singly cultured for 7 days in the presence of SF, IL-6, and IL-3 in 96-well plates. After 7 days of culture, colonies were divided into two aliquots. An aliquot of the colonies was cultured for 7 more weeks in the presence of SF at 100 ng/mL and IL-6 at 50 ng/mL in 96-well plates (plate A). The other aliquot of colonies was cultured for 3 more weeks in the presence of IL-3 at 5 ng/mL in 96-well plates (plate B).