Fig. 1.
C5a stimulation of immunoreactive IL-8 secretion from PBMC. PBMC were incubated with different concentrations (from 10 to 1,000 nmol/L) of recombinant human C5a. After 2 hours, cells were removed and immunoreactive IL-8 in the medium was determined by ELISA. For each stimulation, 0.5 × 106 freshly prepared PBMC were used. Data presented are the mean ± SE from 6 individual donors.
