Analysis of mRNA expression of members of the bcl-2 family. Total RNA of PMN freshly isolated from the circulation, HL-60, or BL41 cells for positive control, respectively, was subjected to RT-PCR using primers specific for bcl-2 or bcl-X (A), as well as for bax- or bak (B). PCR products on a 1.5% agarose gel are shown. Results are representative of three independent experiments.