Expression of BARTs in PBMC. RT-PCR was used to examine BART expression in (A) unfractionated PBMC and CD19⁺-selected PBMC. RPMS1 and BARF0 were detected in CD19⁺-selected PBMC isolated from an individual donor (I) and from pooled blood (P), as well as in unfractionated PBMC from an individual (I). EBV⁻ BJAB cells served as the negative control. (B) CD23⁺ PBMC. RPMS1, BARF0, and EBER1 transcripts were not detected in CD23⁺ PBMC isolated from an individual (I). Detection of CD23 transcripts in the individual PBMC sample was used to illustrate the quality of the mRNA in this sample. Raji was used as a positive control for CD23 RT-PCR.