Verification of the identity of the RPMS1 RT-PCR product. Alignment of an RPMS1 RT-PCR sequence cloned from a CD19⁺PBMC sample to known EBV sequences. The 5′ part of the sequence matched to Raji sequences spanning the B95-8 major deletion and the 3′ part of sequence matched B95-8 sequences as shown. Arrows indicate the splice sites and the numbers represent the positions in the Raji or B95-8 genome. Single-underlined sequences represent the primers used to generate the RT-PCR clone and the double-underlined 5 bp indicates an insertion relative to a reported cDNA.39The splice acceptor site (115,725) is the same as that seen in RK139 and the same sequence as that seen in C22.2,40 although there is a discrepancy in the numbering, which is given as 115,730 in Smith et al.40