RPMS1 protein expression. (A) Western blot analysis of 293T cells transfected with an expression vector containing the Flag-tagged RPMS1 ORF. The Flag-RPMS1 protein band detected using anti-Flag mouse MoAb is indicated by an arrow. The relative positions of the molecular weight markers are shown on the left. Lysate of nontransfected 293T cells was used as the control. (B) Indirect immunofluorescence assay showing Flag-RPMS1 expression in transfected Vero cells. Flag-RPMS1 was detected using anti-Flag MoAb and FITC-conjugated secondary antibody.