Fig. 3.
Fig. 3. Southern blot analysis of TCRβ gene rearrangements. (A) Schematic diagram of the germline configuration of the human TCRβ locus showing the position of the TCRBJ1 and TCRBJ2 probes. (B) Southern blot analysis of filters containing EcoRI–digested DNA of HeLa cells (lane 1), six human thymocyte subpopulations (lanes 2 to 7), and total human thymocytes (lane 8) hybridized with the TCRBJ1 (top panel) and TCRBJ2 (bottom panel) probes. Rearranged bands were assigned based on the size of the bands.21 (C) Rehybridization of the filters with the IGKDE probe was performed as a control for loading of the lanes. G and R indicate germline and as yet unidentified rearrangements, respectively. *Partial resistantEcoRI restriction site in the TCRCB2 region. Underdigestion of this restriction site results in a 7.9-kb germline band.

Southern blot analysis of TCRβ gene rearrangements. (A) Schematic diagram of the germline configuration of the human TCRβ locus showing the position of the TCRBJ1 and TCRBJ2 probes. (B) Southern blot analysis of filters containing EcoRI–digested DNA of HeLa cells (lane 1), six human thymocyte subpopulations (lanes 2 to 7), and total human thymocytes (lane 8) hybridized with the TCRBJ1 (top panel) and TCRBJ2 (bottom panel) probes. Rearranged bands were assigned based on the size of the bands.21 (C) Rehybridization of the filters with the IGKDE probe was performed as a control for loading of the lanes. G and R indicate germline and as yet unidentified rearrangements, respectively. *Partial resistantEcoRI restriction site in the TCRCB2 region. Underdigestion of this restriction site results in a 7.9-kb germline band.

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