Fig. 3.
FACS analyses of CCR-3 expression on fractionated human thymocytes. Thymocytes were prepared as described in Methods and stained with anti-CCR-3 (7B11; IgG2a) according to standard protocols. After secondary antibody labeling, the cells were stained with anti-CD4-PE and anti-CD8-PE-Cychrome. Three-color analyses were performed according to standard methodology on gated subpopulations as shown by dot-plot. The results are representative of n = 3 separate donors.