Eotaxin-induced migration of human thymocytes. (A) Dose-response curve of eotaxin-induced thymocyte migration. Migration assay was performed as previously described.22 Each point represents mean ± SE mean number of migrated cells in 5 high power fields (×400) from n = 5 experiments performed in duplicate. (Inset) Dose-response curve for human recombinant lymphotactin-induced migration, as a positive control, from the same n = 5 experiments performed in duplicate. (B) Eotaxin-induced calcium mobilization in unfractionated Indo-1AM–loaded thymocytes. Fluorimetry was performed as described in Bacon et al23 using a PTI fluorimeter. The trace is representative of n = 5 separate experiments.