Cell-cycle analysis of E-selectin-positive HDMECs. Proliferating (A through D) and confluent (E through H) HDMECs were treated without (Control; A, B, E, and F) or with 0.2 μg/mL LPS (LPS; C, D, G, and H) for 4 hours. Cells were analyzed for E-selectin expression and distribution in G₀/G₁ and S to G₂/M as described in Materials and Methods. (A, C, E, and G) Histograms of DNA content (ie, propidium iodide staining) for the total cell population. (B, D, F, and H) Histograms of DNA content in E-selectin–positive cells (FITC⁺). The percent distribution of cells in different phases of the cell cycle (G₀/G₁, S, G₂/M) is shown for each panel.