Increased E-selectin in bFGF-stimulated HDMECs. Cells were serum-starved and bFGF-starved for 24 hours, trypsinized, and replated at subconfluent density in the absence (gray line) or presence of 2 ng/mL bFGF (solid black region) for 46 hours, and then analyzed by flow cytometry for cell-cycle distribution (A) and E-selectin expression (C and E). Nonspecific antibody binding was measured using isotype-matched control IgG1 (B and D). Forty-six percent of the cells were E-selectin–positive (C) and 27% were positive (E).