Fig. 5.
Fig. 5. HL-60 cell adhesion to HDMEC. HL-60 cell adhesion to endothelial cells treated for 5 hours with PBS (solid bars) or with 0.2 μg/mL LPS (hatched bars) was measured in HDMEC 3 days (proliferating) or 7 days (confluent) after trypsin passage (A). (B) HL-60 adhesion to proliferating HDMEC was measured in presence of 2.5 mmol/L EGTA, 20 μg/mL anti–E-selectin MoAb H18/7, or an isotype-matched IgG2a control MoAb. Cells bound ± SEM was calculated from 10 randomly selected fields.

HL-60 cell adhesion to HDMEC. HL-60 cell adhesion to endothelial cells treated for 5 hours with PBS (solid bars) or with 0.2 μg/mL LPS (hatched bars) was measured in HDMEC 3 days (proliferating) or 7 days (confluent) after trypsin passage (A). (B) HL-60 adhesion to proliferating HDMEC was measured in presence of 2.5 mmol/L EGTA, 20 μg/mL anti–E-selectin MoAb H18/7, or an isotype-matched IgG2a control MoAb. Cells bound ± SEM was calculated from 10 randomly selected fields.

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