Fig. 5.
Inhibition of adhesion of B lymphocytes to Fn by affinity purified anti-RGD antibodies from IVIg. Fn-coated wells were preincubated with rabbit anti-Fn serum, control rabbit antibodies, F(ab′)2 fragments of IVIg eluted from the RGD-affinity column and anti-RGD-depleted F(ab′)2 fragments of IVIg. Raji cells that were pretreated with anti-CD19 MoAb was incubated in triplicates at 1.105 cells per Fn-coated well for 20 minutes at 37°C. After washing, the number of bound cells per well was calculated as the mean number of cells counted in five high-power fields (×400) as described in Materials and Methods.
