American Society of Hematology

Fig. 6.

$Fig. 6. Inhibition of platelet adhesion to vWF and Fg. Wells were coated for 2 hours at 37°C with 10 μg/mL of vWF or Fg. The protein-coated wells were incubated with buffer (A, D, G), anti-RGD depleted Ig fraction (1.3 μmol/L) (B, E, H) or anti-RGD Ig (1.3 μmol/L) (C, F, I) for 1 hour at 37°C. Unstimulated platelets (A-C) and thrombin-stimulated platelets (D-I) were allowed to adhere to Fg-coated wells (A-F) or vWF-coated wells (G-I) for 20 minutes at room temperature. After removal of nonadherent platelets, adherent platelets were fixed, stained, and microphotographed at a 40-fold magnification.$

Inhibition of platelet adhesion to vWF and Fg. Wells were coated for 2 hours at 37°C with 10 μg/mL of vWF or Fg. The protein-coated wells were incubated with buffer (A, D, G), anti-RGD depleted Ig fraction (1.3 μmol/L) (B, E, H) or anti-RGD Ig (1.3 μmol/L) (C, F, I) for 1 hour at 37°C. Unstimulated platelets (A-C) and thrombin-stimulated platelets (D-I) were allowed to adhere to Fg-coated wells (A-F) or vWF-coated wells (G-I) for 20 minutes at room temperature. After removal of nonadherent platelets, adherent platelets were fixed, stained, and microphotographed at a 40-fold magnification.

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