Fig. 4.
![Fig. 4. FPR phosphorylation and MAP kinase activation induced by T20/DP178. FPR phosphorylation was measured by incubating [32P]-orthophosphate–labeled ETFR cells with medium alone or T20/DP178 at 37°C for 15 minutes, and the same amount of cell lysate for each treatment was immunoprecipitated with an antiepitope (anti-HA) antibody (A). MAPK activation was examined in human monocytes by treatment of the cells with different concentrations of T20/DP178 at various time intervals and immunoprecipitation of the cell lysates with an anti-p38 MAPK antibody, followed by immunoblotting with a mouse antiphosphotyrosine antibody (B). In time-course experiments of MAPK activation, 10 μmol/L T20/DP178 was used.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/93/11/10.1182_blood.v93.11.3885/4/blod41119004bw.jpeg?Expires=1735809616&Signature=DaeYtOq6ABggq5-ey70Nh0s~mhuQljxeCj4e9puh2ABT7whCFKxq7x0083cx9cKH3zhrbRXksGl4G2sa4zng2HYBJT7ggsF6GnDs~zxCBaydVYo9RtxH9u4uQ3QtjyNHJ4HWNR7bZnzpiHVeM7eQDb7LmA2kTX5-F594oS674AOjMbjkkMkaN2FEFpIAOhPNMpJtMDqJYuPKiYn-v~Pti93NjXEr6o~Fm3O4qErXcuPU8ATMeRgOurhZATZSPcOMsHE0q6AE8VTdwBH4Iba7baA4~7mAkZXR4T109NfiaeN-cENgZmNezny4XN80hf1hFmS59XP8C-rbrFyEZw7NLw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
FPR phosphorylation and MAP kinase activation induced by T20/DP178. FPR phosphorylation was measured by incubating [32P]-orthophosphate–labeled ETFR cells with medium alone or T20/DP178 at 37°C for 15 minutes, and the same amount of cell lysate for each treatment was immunoprecipitated with an antiepitope (anti-HA) antibody (A). MAPK activation was examined in human monocytes by treatment of the cells with different concentrations of T20/DP178 at various time intervals and immunoprecipitation of the cell lysates with an anti-p38 MAPK antibody, followed by immunoblotting with a mouse antiphosphotyrosine antibody (B). In time-course experiments of MAPK activation, 10 μmol/L T20/DP178 was used.
