Fig. 4.
Inhibition of cell stimulation of plasminogen activation by uPA-PMSF. Increasing amounts of uPA treated with PMSF were incubated in reaction mixtures of 15 pmol/L uPA, 100 nmol/L lys-plasminogen, and 5 × 106 Nalm6 cells/mL. Plasmin generation rate was calculated for increasing levels of uPA-PMSF showing how binding of active uPA to cells is necessary for cell stimulation of kinetics. Rates were determined in triplicate and are expressed as mean ± SE.