Fig. 2.
Jaspamide induces actin aggregation in HL-60 cells in a dose- and time-dependent manner. HL-60 cells were treated with jaspamide and stained for actin (with mouse anti-actin MoAb labeled with rhodamine-conjugated donkey anti-mouse antibody) and analyzed by CLSM. (A) Untreated cells. (B) Cells grown for 24 hours with 10−8 mol/L jaspamide. (C, C’) Cells grown for 24 hours with 10−7 mol/L jaspamide; in C’ the actin staining (red) is overlaid on the Nomarski image (gray). (D) Cells grown for 48 hours in the presence of 10−8 mol/L jaspamide. (E) Cells grown for 48 hours in the presence of 10−7 mol/L jaspamide (A through E: original magnification × 250) (C’: original magnification × 2,100).
