Effect of jaspamide on intracellular movement and ruffling in monocytes. Nomarski time-lapse photography of monocytes treated for 1 hour with (D through F and J through L) 10−7 mol/L jaspamide compared with (A through C, G through I) untreated cells. Cellular movement (A through F). Nomarski images of a 1-second interval of the (A through C) untreated cells exhibiting movement on the substrate (arrow indicating spreading region); (D through F) jaspamide-treated cells that exhibited no significant movement. Ruffling and intracellular movement: (G through L) 0.05-second interval of treated and untreated cells, after (G and J) 10 minutes and (H and K) 10 minutes + 0.05 seconds were analyzed. Using the CLSM program, we determined the cellular movement that occurred in 0.05 seconds by subtracting the 10-minute images from the images of 10 minute + 0.05 seconds (images 5H minus 5G and images 5K minus 5J) for untreated and treated cells, respectively. The resulting calculation of movement is shown as red areas superimposed on 10-minute + 0.05-second untreated and treated images (I and L, respectively) (original magnification × 1,400).
