Effect of the MLL/MEN chimeric protein on p53-independent transcription. HeLaS3 cells were transfected with 2 μg of GAL4-Luc, 2 μg of pME18S-GAL4/VP16, and 2 μg of pME18S-MEN(HA) or pME18S-MLL/MEN(HA) by using a calcium phosphate method and were assayed in the same way as in Fig 1.