Deletion mutants of MEN that disrupt the binding with p53. (A) Cell extracts derived from COS1 cells transfected with CMVp53 and pME18S-MEN(HA) or pME18S-dMEN1-7(HA) were immunoprecipitated with anti-p53 monoclonal antibody. Western blot was performed using anti-HA polyclonal antibody (HA.11). (B) Lanes 1 through 8 contain the equal amount of the TCLs from the same samples used for the immunoprecipitation assays in (A). Western blot was performed using anti-HA polyclonal antibody (HA.11).