Fig. 3.
Fig. 3. Partial CGH karyotypes, corresponding ratio profiles, and Southern blot analysis of different MCLs. Hybridized tumor DNA was labeled with Spectrum Red-dUTP and control DNA with Spectrum Green-dUTP. On the right side of the ideograms, the average ratios of tumor/normal fluorescence are plotted. The central line indicates a ratio value of 1.0; lines on the left side indicate ratio values of 0.75 and 0.5, respectively; lines on the right side indicate ratio values of 1.25 and 1.5, respectively. n = number of chromosomes analyzed for calculating the respective average ratio profile. β-ACTIN probe was used as loading control in all Southern blots. (A) (Left) Partial CGH karyotype of case 5. A reduction of 17p arm is visible. (Right) Southern blot analysis of DNA of the same case (case 5), another case with a similar chromosome 17 profile (case 15), an additional case with normal chromosome 17 profile (case 20), and DNA from lymphocytes of a healthy control. Both cases with 17p loss by CGH (cases 5 and 15) showed homozygous deletions of P53gene. (B) (Left) Partial CGH karyotype of case 5. A gain of 12q13 is visible. (Right) Southern blot analysis of DNA of the same case (case 5), another case with gain of 12q by CGH (case 2), an additional tumor with normal chromosome 12 profile (case 10), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification of CDK4 gene in case 5. (C) (Left) Partial CGH karyotype of case 1. An intense, band-like hybridization signal mapping to chromosomal band 10p12-p13 is observed. (Right) Southern blot analysis of DNA of the same case (case 1), another tumor with a similar profile of chromosome 10 (case 16), two additional tumors with normal chromosome 10 profile (cases 20 and 25), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification ofBMI-1 gene. (D) (Left) Partial CGH karyotype of case 4. An intense, band-like hybridization signal mapping to chromosomal band 8q24 is observed. (Right) Southern blot analysis of DNA of the same case (case 4), two additional tumors with normal chromosome 8 profile (cases 9 and 3), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification of C-MYC gene.

Partial CGH karyotypes, corresponding ratio profiles, and Southern blot analysis of different MCLs. Hybridized tumor DNA was labeled with Spectrum Red-dUTP and control DNA with Spectrum Green-dUTP. On the right side of the ideograms, the average ratios of tumor/normal fluorescence are plotted. The central line indicates a ratio value of 1.0; lines on the left side indicate ratio values of 0.75 and 0.5, respectively; lines on the right side indicate ratio values of 1.25 and 1.5, respectively. n = number of chromosomes analyzed for calculating the respective average ratio profile. β-ACTIN probe was used as loading control in all Southern blots. (A) (Left) Partial CGH karyotype of case 5. A reduction of 17p arm is visible. (Right) Southern blot analysis of DNA of the same case (case 5), another case with a similar chromosome 17 profile (case 15), an additional case with normal chromosome 17 profile (case 20), and DNA from lymphocytes of a healthy control. Both cases with 17p loss by CGH (cases 5 and 15) showed homozygous deletions of P53gene. (B) (Left) Partial CGH karyotype of case 5. A gain of 12q13 is visible. (Right) Southern blot analysis of DNA of the same case (case 5), another case with gain of 12q by CGH (case 2), an additional tumor with normal chromosome 12 profile (case 10), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification of CDK4 gene in case 5. (C) (Left) Partial CGH karyotype of case 1. An intense, band-like hybridization signal mapping to chromosomal band 10p12-p13 is observed. (Right) Southern blot analysis of DNA of the same case (case 1), another tumor with a similar profile of chromosome 10 (case 16), two additional tumors with normal chromosome 10 profile (cases 20 and 25), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification ofBMI-1 gene. (D) (Left) Partial CGH karyotype of case 4. An intense, band-like hybridization signal mapping to chromosomal band 8q24 is observed. (Right) Southern blot analysis of DNA of the same case (case 4), two additional tumors with normal chromosome 8 profile (cases 9 and 3), and DNA from lymphocytes of a healthy control. Densitometric evaluation showed an amplification of C-MYC gene.

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